Coding
Part:BBa_K3286207:Design
Designed by: Ben Kuipers Group: iGEM19_Wageningen_UR (2019-10-20)
RpfCch (TMD) sfGFP fusion
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 227
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To fuse these parts, the N- terminal part of X. fastiosa RpfC (basepairs 1- 516) was used, and the startcodon of the sfGFP was removed. The parts were fused with Golden Gate.
Source
See the BBa_K3286200, BBa_K3286203 parts for further information on part source.