Coding

Part:BBa_K3286207:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)


RpfCch (TMD) sfGFP fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fuse these parts, the N- terminal part of X. fastiosa RpfC (basepairs 1- 516) was used, and the startcodon of the sfGFP was removed. The parts were fused with Golden Gate.


Source

See the BBa_K3286200, BBa_K3286203 parts for further information on part source.

References